Extended Data Fig. 3: Gating strategy of TH cell populations in vivo. | Nature Immunology

Extended Data Fig. 3: Gating strategy of TH cell populations in vivo.

From: An αvβ3 integrin checkpoint is critical for efficient TH2 cell cytokine polarization and potentiation of antigen-specific immunity

Extended Data Fig. 3

Extended Data Fig. 3(a) & (b) Flow cytometric gating strategy for cytokine- and transcription factor- expressing THeff cells in the mediastinal lymph node of OVA/Alum-challenged mice. (c) Flow cytometric gating strategy for 2W1S-tetramer-specific TH2 cells in the papain-challenged lung. (d) & (e) Flow cytometric gating and quantification of 2W1S-tetramer-specific IL-5 and IL-13 producing TH effector cells in PMA/ionomycin stimulated lung lymphocytes. Data are pooled from 2 independent experiments and represent mean ± SD (n = 6 mice in naïve and papain only control groups, n = 16 mice in Cd4Cre group, n = 15 mice in ItgavCD4KO group); unpaired two-sided t-test; *P = 0.0418 (top, IL-5) and 0.0211 (bottom, IL-13). (f) Flow cytometric gating strategy for IFN-γ expressing THeff cells in the mediastinal lymph node of LPS-challenged mice.

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