Extended Data Fig. 8: E8i-driven Cre recombinase expression efficiently deletes CD39 on CD8+ TIL.
From: Hypoxia drives CD39-dependent suppressor function in exhausted T cells to limit antitumor immunity
(a) CD39 expression in day 14 B16-F10 tumor-infiltrating CD8+ T cells from Entpd1f/f or E8iCre-ERT2Entpd1f/f mice. (b) Cytokine production following PMA/Ionomycin re-simulation of day 14 CD8+ TIL. (c, d) Infiltration of adoptively transferred pmel-I T cells into tumor-draining lymph nodes (dLN; C) and (D) quantification of pmel-I percentages in a representative experiment. (e) Ex vivo cytokine production of pmel-I T cells following gp100 re-stimulation. (f) TIL analysis of CD39 expression in CD8+ T cells from E8iCre-ERT2Entpd1f/f mice treated for three consecutive days with tamoxifen. (g, h) Representative flow cytometry plots from Fig. 7e, f. Statistics are one-way ANOVA (B,D,E) and Mann-Whitney (F) with ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001.
