Fig. 5: SHP-2 deletion increases monocyte and DC specification gene transcripts and imprints an effector differentiation program in TAMs.
From: SHP-2 and PD-1-SHP-2 signaling regulate myeloid cell differentiation and antitumor responses
a,b, Volcano plot of DE genes (a) and heat map (b) of the top 6,500 DE genes in TAMs isolated from MC17-51 tumor-bearing Shp2f/f and Shp2f/fLysMCre mice and analyzed by RNA-seq. log2(FC) > 0 and log2(FC) < 0 for upregulated and downregulated genes, q < 0.05 for all DEGs, respectively). c, Expression of the indicated genes by TAMs from Shp2f/f and Shp2f/fLysMCre tumor-bearing mice (data from RNA-seq dataset). d, Bubble plot of substantially enriched pathways (q < 0.1) in TAMs of Shp2f/fLysMCre mice sorted by GeneRatio. e, Heat maps of differentially expressed genes related to macrophage differentiation, macrophage activation, phagocytosis and TLR signaling in TAMs from Shp2f/f and Shp2f/fLysMCre tumor-bearing mice (q < 0.05). f,g, Quantification of IRF8, IFN-γ, CD86 and IL-10 expression (f) and representative flow cytometry of IRF8 and IFN-γ expression (g) in TAMs of tumor-bearing Shp2f/f and Shp2f/fLysMCre mice. Results are representative of four independent experiments with four to six mice per group. h,i, Bubble plot of cytokine pathways (h) and metabolism pathways (i) substantially enriched in TAMs (q < 0.1) from Shp2f/fLysMCre mice sorted by GeneRatio.
