Extended Data Fig. 1: Myeloid-specific SHP-2 depletion diminishes tumor growth.
From: SHP-2 and PD-1-SHP-2 signaling regulate myeloid cell differentiation and antitumor responses
a, Shp2f/f, Shp2f/fLckCre, and Shp2f/fLysMCre mice were inoculated with MC17-51 fibrosarcoma cells, tumor volume was monitored longitudinally, and comparisons were made on each day of assessment. Data shown are means of n = 6 mice per group and are representative of three independent experiments. (*p < 0.05, **p < 0.01), ANOVA. b, Representative images of tumors isolated at day 15 from each of the three experimental group are shown. c, d, Shp2f/f and Shp2f/fLysMCre mice were inoculated with B16-F10 melanoma cells, tumor volume was monitored longitudinally (c) and tumor weight was measured at termination on day 16 (d). Data are means of n = 5 mice per group and are representative from one of four independent experiments. (*p < 0.05, **p < 0.01), unpaired t-test two tailed. e-g, The frequencies of CD45+CD11b+ myeloid cells (e), the fractions of macrophages, PMN-MDSC and M-MDSC (f) and the ratio of M-MDSC/PMN-MDSC (g) in tumors were assessed. Data are representative of means ± SD are shown. Results from one representative of 4 independent experiments with n = 4 mice per group are shown (*p < 0.05, **p < 0.01), unpaired t-test two tailed.
