Extended Data Fig. 3: YTHDF2 deficiency in myeloid cells does not affect phagocytosis by macrophages and CD4⁺ T and NK cells in the tumor microenvironment.

a, b, Median fluorescence intensity (MFI, a) and representative histogram (b) of pHrodo red zymosan bioparticles uptake by anti-tumoral BMDMs from Ythdf2^f/f and Ythdf2^cKO mice (n = 3). c, B16-OVA tumor growth on day 18 post-B16-OVA tumor inoculation in Rag1^−/− mice s.c. transplanted with BMDMs from Ythdf2^f/f and Ythdf2^cKO mice (n = 3). d, B16-OVA tumor growth on day 13 post-B16-OVA tumor inoculation in Rag1^−/− mice that were i.v. injected with CD3⁺ T cells and s.c. transplanted with BMDMs from Ythdf2^f/f and Ythdf2^cKO mice (n = 5). e-h, Percentages (e, g) and absolute numbers (f, h) of tumor-infiltrating CD4⁺ T cells, CD8⁺ T cells, and CD3⁻NK1.1⁺ NK cells from Ythdf2^f/f and Ythdf2^cKO mice on day 14 post-B16-OVA tumor cell implantation (e, f) or MC38 (g, h) tumor inoculation (n = 5). i, j, Percentages and representative plots of tumor-infiltrating CD4⁺ IFN-γ⁺ T_H1 cells, CD4⁺ IL-17A⁺ T_H17 cells, CD4⁺ Foxp3⁺ Treg cells, and granzyme B-producing NK cells from Ythdf2^f/f and Ythdf2^cKO mice on day 14 post B16-OVA (i) or MC38 (j) tumor inoculation (n = 4). Data are shown as mean ± SD and were analyzed with an unpaired two-tailed t-test (a, e-j) or two-way ANOVA with mixed-effects model and adjusted by the Holm-Šídák post-test (c, d). Data in a-j are representative of at least two independent experiments. NS, not significant.

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