Extended Data Fig. 8: Differential impacts of sTNF and tmTNF on early T cell precursor generation.
a, Overlays of the UMAP for cells derived from specific (red: control, tmTNF or sTNF) and all conditions (gray). b, UMAP visualization of the cell cycle status for all 26 cellular clusters shown in Fig. 7a. c, The enrichment scores of IFN-related gene signature for all the annotated populations of hematopoietic cells shown in Fig. 7b. d, The relative distribution of cells, derived from ATOs without and with TNF stimulus, in cluster 4 and 15 that are annotated as lymphoid progenitors. e, Heatmap shows the average expression of IFN-related genes by the previously annotated populations of human CD34+ thymocytes. f-l, Scheme illustrates the experimental design where CD7+ T-specified progenitors, derived from control or TNF activated (sTNF at 0.25 ng/mL or tmTNF at 1% density)-ATOs assembled with cord blood lin−CD34+ HSPCs, were examined for their maturation potential towards later stages of T-lineage development (f). Frequency (g) and cell count (h) of CD4+CD8b+ thymocytes (n = 5) that were generated from the secondary ATO cultures. Frequency (i) and cell count (j) of CD3+TCRαβ+ T cells (n = 5) that were generated from the secondary ATO cultures. Frequency (k) and cell count (l) of CD3+TCRγδ+ T cells (n = 5) that were generated from the secondary ATO cultures. n, biological replicates (g-l).
