Extended Data Fig. 6: Phenotype of CD8 T cells in IFNγ∆KRKR mice after LCMV infection.
From: IFNγ binding to extracellular matrix prevents fatal systemic toxicity
IFNγ∆KRKR and IFNγwt mice were infected i.v. with LCMV-Docile and analysed between day 10-13. a, splenocyte counts and b, CD8 T cells within splenocytes. c, GP33-specific cells within CD8 T cells. d, NP396-specific cells within CD8 T cells. e, Differentiation of effector CD8 T cells according to their expression of KLRG-1 and CD127 (exemplary staining shown in the upper two panels) into “EECs” (early effector cells), “SLECs” (short lived effector cells) and “MPECs” (memory precursor effector cells) was followed. f, Based on CD62L and CD44 expression (exemplary staining shown in the upper two panels), naïve CD8 T cells were discriminated from effector cells with a Tcm (central memory) or Tem (effector memory) phenotype. g, PD-1 and LAG3 expression (exemplary staining shown in the upper two panels) of CD8 T cells after in vitro restimulation with GP33 or NP396 peptides. h, IFNγ expression (exemplary staining shown in the upper two panels) of CD8 T cells after in vitro restimulation with GP33 or NP396 peptides. In b-h, always relative frequencies (%) as well as absolute cell numbers (#) are shown as individual mice, mean and s.d.. IFNγΔKRKR (n = 11) and IFNγwt (n = 17) mice from four independent experiments are shown. Significance in a-h was calculated using the Mann-Whitney test (two-sided) with n.s. not significant, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
