Extended Data Fig. 3: Generation of IFNγΔKRKR mice.
From: IFNγ binding to extracellular matrix prevents fatal systemic toxicity
a, A PCR fragment of exon 4 of IFNγ from CRISPR/Cas9-modified founder mice was sequenced. Sequences were aligned to the native IFNγ sequence (C57BL/6 N, NC_000076.6) and the repair template to validate deletion events. Three out of nine mice are shown. b, The deletion of an AciI restriction site within the KRKR motif indicates zygosity in founder mice. Upper panel shows PCR product, lower panel AciI digestion. The 480 bp fragment indicates homozygous deletion of 12 bp encoding the KRKR motiv. Each lane corresponds to one founder, each founder was genotyped at least twice. The C57BL/6 N mouse #2552 was used as a wild type control (wt).
