Extended Data Fig. 3: Multiple recurrent prostate-infiltrating CD4+ Tconv clones exhibit hallmarks of steady-state activation.
Three Group 1 and four Group 3 TCRs were expressed as TCRrg mice. >6 weeks after bone marrow reconstitution, TCRrg T cells were directly phenotyped using flow cytometry. a, Summary plot of data from Fig. 2a, b showing the percentage of CD69 expression by Group 1 (black symbols) and Group 3 (gray symbols) TCRrg T cells (TCRβ+CD4+Thy1.1+) isolated from the indicated regional lymph nodes of primary TCRrg mice (n = 1–7 per TCRrg). Data are pooled from eleven independent experiments. Each symbol depicts cells from an individual TCRrg mouse. iLN, inguinal lymph nodes; aLN, axillary lymph nodes; bLN, brachial lymph nodes; cLN, cervical lymph nodes; pLN, para-aortic lymph nodes; mLN, mesenteric lymph nodes. b, Representative flow cytometric analysis of Ki67 expression by Group 1 (top) and Group 3 (bottom) TCRrg T cells (TCRβ+CD4+Thy1.1+) isolated from the spleen of primary TCRrg mice. SP, spleen. c, Summary plot of data from b showing the percentage of Ki67 expression by Group 1 (black symbols) and Group 3 (gray symbols) TCRrg T cells (TCRβ+CD4+Thy1.1+) isolated from the spleen or pooled lymph nodes of primary TCRrg mice (n = 1–8 per TCRrg). Data are pooled from eight independent experiments. Each symbol depicts cells from an individual TCRrg mouse. SP, spleen; LN, pooled lymph nodes (which include inguinal, axillary, brachial, cervical, para-aortic, and mesenteric lymph nodes). Bold horizontal lines represent means. Error bars represent means ± s.e.m. Two-sided p values were calculated using Student’s t-tests for pooled Group 1 clones versus pooled Group 3 clones. ns, p ≥ 0.05. ns, not significant. Source data contain exact p values and group sizes.
