Extended Data Fig. 6: The frequency and phenotype of Group 3 SAS T cells is not impacted following transfer to B cell-depleted recipients.
The Group 1 ANT TCR and Group 3 SAS TCRs were cloned and expressed as TCRrg mice. 1 × 105 FACS-purified TCRrg cells were transferred into treated recipient mice. 7 d prior to TCRrg cell transfer, recipient mice were treated with anti-CD20 or control antibody (see Methods). Donor cell frequency and phenotype was assessed 7 d post-transfer. a, Representative flow cytometric analysis showing the efficiency of B cell depletion in control and anti-CD20 treated recipient mice. SP, spleen; LN, pooled lymph nodes; PP, Peyer’s Patches. b, Summary plot of data from a showing the frequency of B cells (CD19+TCRbneg) recovered from the indicated sites of control- and anti-CD20-treated recipient mice (n = 10–12 per condition). Data pooled from two independent experiments. Each symbol depicts cells from an individual TCRrg mouse. c, Representative flow showing the frequency of CD45.2+CD45.1neg donor TCRrg cells recovered from the spleen of control- and anti-CD20-treated recipient mice. d, Summary plot of data from c showing the frequency of ANTrg and SASrg donor T cells in the spleen of recipient mice treated with control or anti-CD20 antibody (n = 5–7 per condition). Data pooled from two independent experiments. e, Representative flow analysis of donor TCRrg T cells expressing the indicated TCRs in either control or anti-CD20 treated recipients. Histograms of expression of the indicated markers are shown for the indicated TCR and treatment group. The percentage of cells falling within the indicated gates is denoted. f, Summary plots of data from e. For control treated mice and anti-CD20 treated mice, the percentage of cells expressing the indicated marker is plotted for the indicated TCR (n = 5–7 per condition). Data pooled from two independent experiments. Bold horizontal lines represent means. Error bars represent means ± s.e.m. Two-sided p values were calculated using unpaired Student’s t-tests and corrected for multiple comparisons using the Holm-Šídák method. ns, p ≥ 0.05, not significant. Source data contain exact p values and group sizes.
