Extended Data Fig. 2: Representative flow cytometric analysis of TCRrg mice expressing Group 1 vs. Group 3 TCRs.

Two Group 1 TCRs (ANT and DAS) and two Group 3 TCRs (SAS and SKV) were expressed as TCRrg mice. >6 weeks after bone marrow reconstitution, TCRrg T cells were directly phenotyped using flow cytometry. a, Representative gating strategy schematic and flow cytometric analysis of Foxp3, CD44, and CD69 expression by splenic Group 1 (top) and Group 3 (bottom) TCRrg T cells (top row, TCRβ⁺CD4⁺Thy1.1⁺) or respective host T cells (TCRβ⁺CD4⁺CD45.1⁺). T cells were selected by gating on TCRβ⁺B220^neg, followed by CD4⁺CD8β^neg, then by Thy1.1⁺CD45.1^neg (for TCRrg T cells) or Thy1.1^negCD45.1⁺ (for host T cells). b, Summary plot of data from a showing the frequency of splenic Group 1 (black symbols) and Group 3 (gray symbols) TCRrg T cells (TCRβ⁺CD4⁺Thy1.1⁺) within TCRβ⁺CD4⁺ gate. Summary plot of data from a showing the frequency of B cells (B220⁺) within CD45.1⁺ gate. Summary plot of data from a showing the frequency of host CD8α⁺ cells (CD45.1⁺CD8α⁺) within CD45⁺ gate. Summary plot of data from a showing the frequency of host CD4⁺ cells (CD45.1⁺CD4⁺) within CD45⁺ gate. Summary plot of data from a showing the frequency of Treg cells (Foxp3⁺) within host CD4⁺ gate. Summary plot of data from a showing the percentage of host Tconv cells (CD45.1⁺CD4⁺Foxp3^neg) expressing CD69. Summary plot of data from a showing the percentage of host Tconv cells (CD45.1⁺CD4⁺Foxp3^neg) expressing CD44. Summary plot of data from a showing the percentage of host CD8α⁺ cells (CD45.1⁺CD8α⁺) expressing CD69. Summary plot of data from a showing the percentage of host CD8α⁺ cells (CD45.1⁺CD8α⁺) expressing CD44. n = 3–4 per TCRrg. Data are pooled from two independent experiments. Each symbol depicts cells from an individual TCRrg mouse. Bold horizontal lines represent means. Error bars represent means ± SEM. p values were calculated using Student’s t-test (two-tailed) for pooled Group 1 clones versus pooled Group 3 clones. ns, p ≥ 0.05, not significant. Source data contain exact p values and group sizes.

Source data