Extended Data Fig. 4: FAO supports CD40-stimulated epigenetic reprogramming.
(a) Proinflammatory cytokines production in Cas9-expressing BMDMs harboring control sgRNA (Ctrl) and ACLY sgRNAs (ACLY sgRNA-1 and -2) stimulated with or without FGK45 mAb. Representative data from three individual experiments (n = 3 per group). (b) Representative flow plot (left) and quantitative results (right) of ACLY expression in TAMs isolated from tumor-bearing mice transplantate with indicated bone marrows. Representative data from two individual experiments and dots represent individual mouse (n = 8 mice per group). (c) qPCR analysis of mRNA expression of anti-inflammatory marker genes in TAMs isolated from indicated tumor-bearing treated with control vehicle (PBS) or FGK45 mAb. Representative data or pooled data from two individual experiments (n = 7 for Ctrl-PBS, n = 5 for Ctrl-FGK45, n = 8 for ACLY gRNA-PBS and n = 7 for ACLY gRNA-FGK45). (d) Metabolic tracing analysis of 13C-labeled palmitate (PA) in BMDMs stimulated with control vehicle or FGK45 mAb for 3 h. M2 + citrate and other indicated TCA cycle metabolites in the BMDMs of indicated groups were determined by mass spectrometry. Culmulative data of three individual experiments (n = 5 per group). All data are presented as mean ± SD and analyzed by a two-tailed Student’s t-test.
