Extended Data Fig. 7: Metabolic fate of glutamine upon CD40 stimulation.
(a) Metabolic tracing analysis of 13C-labeled glutamine in BMDMs stimulated with control vehicle or FGK45 mAb for 0 h or 3 h. Indicated labeled metabolites in the BMDMs of indicated groups were determined by mass spectrometry. (b) The percentage of 13C labeled acetylated histone H3-K27 (Ac-H3K27) in 13C-labeled glutamine pulsed BMDMs stimulated with or without FGK45 mAb was determined by proteomics. The basal labeling level of 13C labeled acetylated histone H3-K27 was determined by the same approach in BMDMs treated with FGK45 mAb plus ACLY inhibitor. Data are presented as mean ± SD. (c) Illustration of metabolic reprogramming induced by CD40 signal in macrophages. Representative data from two individual experiments (n = 3 per group). All data are presented as mean ± SD and analyzed by a two-tailed Student’s t-test.
