Extended Data Fig. 2: CD40 signal reprogram macrophage OXPHOS and mitochondrial activity.
(a) Quantitative result of mitochondrial membrane potential in TAMs isolated from tumor-bearing mice treated with or without FGK45 mAb, which was determined by tetramethylrhodamine (TMRM). Dots represent individual mouse. (b) Quantitative result of mitochondrial membrane potential in BMDMs stimulated with or without FGK45 mAb, which was determined by tetramethylrhodamine (TMRM). (c) Mitochondrial mass and membrane potential in BMDMs stimulated with or without FGK45 mAb were determined by MitoTracker Green (MG) and tetramethylrhodamine (TMRM), respectively. The representative flow plots (left) and the quantitative result of MDR to MG ratio (right) in. (d–e) Extracellular lactate levels (d) and the production of proinflammatory cytokines (e) in BMDMs stimulated with or without FGK45 mAb or LPS under glucose-replete or -deplete conditions were determined by ELISA. (a) Data are representative of two independent experiments. Dots represent individual mouse (n = 6 mice per group). (b–e) Data are representative of three independent experiments (n = 3 per group). All data are presented as mean ± SD and analyzed by a two-tailed Student’s t-test.
