Extended Data Fig. 3: Pyrimidine starvation confines antigen-specific T cells to the splenic T cell zone. | Nature Immunology

Extended Data Fig. 3: Pyrimidine starvation confines antigen-specific T cells to the splenic T cell zone.

From: Pyrimidine de novo synthesis inhibition selectively blocks effector but not memory T cell development

Extended Data Fig. 3

Mice were engrafted with a low number of CD45.1+ congenic OT-1 T cells, infected with Lm-Ova, and treated with (Lefl) and without Leflunomide (Ctrl). Spleens were harvested 7 days post infection. A, upper panels show splenic sections stained with B220 (blue), CD3 (green), and CD45.1 (red). Lower panels show vectored images, which display the localization of individual OT-1 in red. B, the graphs show the relative distribution of OT-1 in the three anatomical locations and the total OT-1 numbers per mm2 in the indicated anatomical location. Data points represent individual mice, center line shows the mean. Data are representative of n = 3 (Ctrl) and n = 3 (Lefl) mice. Two-tailed, unpaired t-tests were performed to calculate significance with *p < 0.05, ** p < 0.01, and ns=not significant (p > 0.05).

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