Extended Data Fig. 9: Single-cell transcriptomic data of leflunomide treated and untreated cells. | Nature Immunology

Extended Data Fig. 9: Single-cell transcriptomic data of leflunomide treated and untreated cells.

From: Pyrimidine de novo synthesis inhibition selectively blocks effector but not memory T cell development

Extended Data Fig. 9

The analysis relates to Fig. 5. A, heatmap depicting the cluster specific expression of selected genes among the clusters. B, tSNE plots with overlaid expression of predicted upstream regulators in red. Each dot represents a cell. Bar graphs show the percentage of cells expressing the respective upstream regulators in each predicted cluster. C, plots depict an enrichment analysis for gene sets controlled by upstream regulators. p-values were adjusted using Benjamini & Hochberg method. D, heatmap shows the cluster resolved activity of the top 30 upstream regulators. Upstream regulators were in both cases predicted by Ingenuity Pathway Analysis (QIAGEN IPA).

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