Extended Data Fig. 10: Reproduction of single-cell transcriptomic data after leflunomide treated.
A and B, louvain clusters depicted in the reduced space calculated by UMAP for control (Ctrl) and leflunomide (Lefl) treated mice from 4 biological replicates each. Overlays of all 4 replicates (A) and individual sample (B) data are presented. C, frequencies of the respective clusters of OT-1 T cells defined in A within in the total CD8+ population. D, frequencies of cells expressing the indicated genes (circle size) and their respective scaled expression levels (color intensity) in each cluster. E, Dhodh expression levels depicted over the reduced space calculated by UMAP for control (Ctrl) and leflunomide (Lefl) treated mice. F, frequency histogram representing the distribution of Dhodh expression levels in cells from control (Ctrl) and leflunomide (Lefl) treated mice. G, developmental trajectories for effector and memory branches depicted over the diffusion map for each condition. Cells are colored according to the clustering. Each dot in A, B, E and G represents a cell. Symbols in C represent individual mice, n = 4 mice per group, and the conditions in C were compared for each cluster using two-tailed, unpaired t-tests with *p < 0.05, **p < 0.01, and ns=not significant (p > 0.05).
