Extended Data Fig. 3: Correlation analyses of chemokine antibodies.
From: Autoantibodies against chemokines post-SARS-CoV-2 infection correlate with disease course
(a) ELISA showing RBD IgG antibodies as OD450 of serial plasma dilutions (top panel) and as AUC (bottom panel) in healthy controls (Control, n = 23) and COVID-19 convalescents (COVID-19, n = 71) from the Lugano cohort at month 6. Average of two independent experiments. Horizontal bars indicate median values. Two-tailed Mann–Whitney U-tests. (b) Neutralizing activity against SARS-CoV-2 pseudovirus shown as relative luciferase units (RLU) normalized to no plasma control (top panel) and half-maximal neutralizing titers (NT50, bottom panel) in healthy controls (n = 9) and in COVID-19 convalescents (n = 71) from the Lugano cohort at month 6. Average of two independent experiments. Horizontal bars indicate median values. Two-tailed Mann–Whitney U-tests. (c) Two-tailed Pearson correlations of RBD IgG and NT50 values to each other and with age. Average of two independent experiments. (d) Two-tailed Pearson correlations of CCL19, CCL22, CXCL17 antibodies and of the cumulative signal of the 42 chemokine antibodies with RBD IgG, NT50 values and age. (e) AUC of ELISA showing CCL19, CCL22 and CXCL17 antibodies in healthy controls (n = 23) and COVID-19 convalescents (n = 71) from the Lugano cohort at month 6 grouped by gender (n = 33, females; n = 38, males). Data are shown as average of two independent experiments. Horizontal bars indicate median values. Kruskal-Wallis test followed by Dunn’s multiple comparison test.
