Extended Data Fig. 4: CSDS-induced changes in T cells and social avoidance in female mice.
From: Dectin-1 signaling on colonic γδ T cells promotes psychosocial stress responses
a, Schematic representation of chronic stress paradigm for female mice. b,c, Attack number (b) and latency (c) of CD-1 aggressors on male versus female mice. n = 100 (ten mice × 10 d) per group. d,e, Attack number (d) and latency (e) of CD-1 aggressors during the proestrus (n = 20), estrus (n = 33), metestrus (n = 18) and diestrus (n = 29) cycle of female mice. f, The percentage of CD-1 behaviors directed towards female mice over the 10-d chronic stress period (left) and over the course of the estrous cycle (middle and right). g, Time the mice spent in the chamber with an inanimate object versus a stranger female mouse (top left). Time the mice spent sniffing an inanimate object versus a stranger female mouse (top right). The color in the dots represents the estrous cycle of the test female mouse. Representative heatmaps depict mice movements (bottom). Control, resilient and susceptible mice (n = 5, 5, 5). h, Percentage of γδ T cells in CD3+ T cells, γδ17 T cells in γδ T cells, CD3+ T cells in viable cells, CD4+ T cells in CD3+ T cells, CD8+ T cells in CD3+ T cells, TH17 cells in CD4+ T cells and Treg cells in CD4+ T cells in the meninges of control, resilient and susceptible female mice (n = 5, 5, 5). b,g, *P < 0.05 and **P < 0.01 (b, P < 0.0001; g, time spent in chambers: P = 0.0039 and P = 0.0033; time spent sniffing: P = 0.0012, P = 0.0237 and P = 0.0049), determined using an unpaired two-tailed Student’s t-test. h, *P < 0.05 (percentage γδ T cells: P = 0.0158, P = 0.0126; percentage γδ17 T cells: P = 0.0203), determined by one-way ANOVA with Tukey’s post-hoc test. Error bars represent the mean ± s.e.m.
