Extended Data Fig. 8: The effects of genetic deletion of dectin-1 and treatment with pachyman on CSDS-induced phenotypes in colonic non-γδ T lymphocytes and sucrose preference test.
From: Dectin-1 signaling on colonic γδ T cells promotes psychosocial stress responses
a–e, Percentage of CD3+ T cells in viable cells (a), CD4+ T cells in CD3+ cells (b), CD8+ T cells in CD3+ cells (c), TH17 cells in CD4+ cells (d) and Treg cells in CD4+ cells (e) in the LP of the colon of WT + ctr, WT + CSDS, dectin-1 KO + ctr and dectin-1 KO + CSDS groups of mice (n = 5, 5, 5, 5). f,g, The average of total fluid consumption (f) and the average percentage of sucrose consumption when given a choice between 1.5% sucrose and water (g) on days 5–8 for WT + ctr, WT + CSDS, dectin-1 KO + ctr and dectin-1 KO + CSDS groups of mice (n = 9, 9, 8, 9). h, Schematic of the experimental design. Eight-week-old TCRd-KO mice received i.v. injection of γδ T cells collected from Clec7a−/− (dectin-1 KO) mice or their WT littermates 1 d before and 5 d after the start of CSDS, followed by a social interaction test (SIT) and tissue collection. i, Schematic of the experimental design. Eight-week-old C57BL/6 mice were orally treated with the vehicle or pachyman daily during the CSDS period, followed by SIT and sucrose preference test (SPT; left). An independent cohort of mice was subjected to tissue harvest 1 d after CSDS (right). j–n, Percentage of CD3+ cells in viable cells (j), CD4+ cells in CD3+ cells (k), CD8+ cells in CD3+ cells (l), TH17 cells in CD4+ cells (m) and Treg cells in CD4+ cells (n) in the LP of the colon of ctr + vehicle, CSDS + vehicle, ctr + pachyman and CSDS + pachyman groups of mice (n = 5, 6, 6, 5). o,p, The average of total fluid consumption (o) and the average percentage of sucrose consumption when given a choice between 1.5% sucrose and water (p) on days 5–8 for four groups of mice: ctr + vehicle, CSDS + vehicle, ctr + pachyman and CSDS + pachyman (n = 10, 10, 10 and 10). *P < 0.05 and **P < 0.01 (c, P = 0.0429; g, P < 0.0001 and P < 0.0001; l, P = 0.0252; p, P < 0.0001 and P < 0.0001), determined by two-way ANOVA with Tukey’s post-hoc test. Error bars represent the mean ± s.e.m.
