Extended Data Fig. 5: Nlrp10 inflammasome is activated upon induction of DSS colitis.
From: Epithelial Nlrp10 inflammasome mediates protection against intestinal autoinflammation
(a) A representative Immunoblot of pro-caspase-1 (p45), cleaved caspase-1 (p20), pro-IL-18 and cleaved IL-18 (n = 6), (b) IL-18 ELISA of supernatants of distal colon explants (n = 6) in Nlrp10D/D and Nlrp10loxP/loxP littermate mice at day 4 after DSS initiation (Unpaired t-test, P = 0.073). (c) Densitometry quantification of cleaved IL-18/pro-IL-18 (Mann-Whitney U test, *P = 0.0286) (d) and cleaved IL-18/actin (Unpaired t-test. *P = 0.0303) in distal colons of Nlrp10D/D and Nlrp10loxP/loxP littermate mice at day 10 after DSS initiation (corresponding to Fig. 3d) (n = 4). (e) A representative immunoblot and (f) quantification (Mann-Whitney U test) (f) of cleaved IL-1β in distal colons of Nlrp10D/D and Nlrp10loxP/loxP littermate mice at day 10 after DSS initiation (n = 4). (g) A representative immunoblot and (h) quantification (Unpaired t-test) of cleaved GSDMD in distal colons of Nlrp10D/D and Nlrp10loxP/loxP littermate mice at day 10 after DSS initiation (n = 4). GSDMD: gasdermin D. Numbers labeled in (a), (e) and (g) represent individual mice in each group. Mean ± SEM. *: p < 0.05. Statistical test used in (b- d), (f) and (h) was two-sided.
