Extended Data Fig. 7: Debio025 and NIM811 inhibit thapsigargin- but not m-3M3FBS-induced NLRP10 activation.
From: Mitochondrial damage activates the NLRP10 inflammasome
(a, d) ASC specks per nuclei in HEK cells expressing NLRP10 and ASCTagBFP, stimulated with m-3M3FBS (85 μM) or thapsigargin (20 μM), in the presence or absence of Debio025 (10 μM) (a) or NIM811 (10 μM) (d) (n = 3). P values were calculated by 2-way ANOVA with Šídák’s multiple comparisons test. *P = 0.0276, **P = 0.0038, ns P = 0.0778-0.0792. (b, e) ASC specks per nuclei in iMacs expressing NLRP3 and ASCmCerulean, stimulated with nigericin (10 μM), poly-(dA:dT) dsDNA (2 μg/ml), m-3M3FBS (85 μM) or thapsigargin (20 μM), in the presence or absence of Debio025 (10 μM) (b) or NIM811 (10 μM) (e) (n = 3). P values were calculated by 2-way ANOVA with Šídák’s multiple comparisons test. ***P = 0.0002, ****P < 0.0001, ns P = 0.2813-0.9999. (c, f) IL-1β secretion from WT iMacs, primed with LPS (200 ng/ml) and stimulated as in (b, e), in the presence or absence of Debio025 (10 μM) (c) or NIM811 (10 μM) (f) (n = 3). P values were calculated by 2-way ANOVA with Šídák’s multiple comparisons test. **P = 0.0027, ****P < 0.0001, ns P = 0.1174-0.9996. Individual data points are means of technical duplicates; all error bars represent SD.
