Extended Data Fig. 3: NLRP10 colocalizes with mitochondria and nucleates ASC specks upon thapsigargin and SMBA1 treatments. | Nature Immunology

Extended Data Fig. 3: NLRP10 colocalizes with mitochondria and nucleates ASC specks upon thapsigargin and SMBA1 treatments.

From: Mitochondrial damage activates the NLRP10 inflammasome

Extended Data Fig. 3: NLRP10 colocalizes with mitochondria and nucleates ASC specks upon thapsigargin and SMBA1 treatments.

(a) Representative confocal micrographs of HEK cells stably expressing NLRP10mCherry and transfected with TOMM20mCitrine mitochondrial marker, stimulated with thapsigargin (20 μM) or SMBA1 (50 μM) (n = 4). (b) ASC specks per nuclei in HEK cells expressing NLRP10 and ASCTagBFP, stimulated with m-3M3FBS (85 μM), thapsigargin (20 μM) or increasing doses of SMBA1 (5, 10, or 50 μM) (n = 3). Individual data points (where applicable) are means of technical duplicates (b); all error bars represent SD.

Source data

Back to article page