Extended Data Fig. 5: Treg cells maintain stability during chronic infection.
From: IFNγ-induction of TH1-like regulatory T cells controls antiviral responses
a, DE of genes induced (upper), or repressed (lower), by Foxp3 in splenic Treg cells of Foxp3Cre-YFP mice compared to Ifngr1L/LFoxp3Cre-YFP mice, that remained uninfected (n = 3) or were infected with Cl. 13 (n = 5) was measured by scRNA-seq. b, c Foxp3Cre-ERT2-GFPRosa26LSL.tdTom mice were treated with tamoxifen for 7d and then remained uninfected or were infected with Cl. 13. b, Experimental schema. c, Representative flow cytometry plot of tdTom and GFP co-expression in splenic CD4+ T cells at steady state (n = 7) or at 16 (n = 5, 2 independent experiments) and 35 (n = 11, 2 independent experiments) dpi with Cl. 13. d-f, Foxp3Cre-YFP or Ifngr1L/LFoxp3Cre-YFP mice were infected with Cl. 13 and levels of Foxp3 (d) (n = 9, control; n = 10, Ifngr1-deficient) and percentage of Nrp1 (e) (n = 9, control; n = 8, Ifngr1-deficient) CD25 (f, left) (n = 11) and level of CD25 (f, right) (n = 11) by splenic Treg cells were determined by flow cytometry. a, c-f, Data are presented as mean values and represent biologically independent mice and 2 (a, d-f and as indicated) or 3 (c) independent experiments. Statistical significance was determined by an unpaired two-tailed Student’s t-test relative to Foxp3Cre-YFP mice (d-f). Adjusted P value was determined by Wilcoxon rank-sum test (a) (P value indicated when significant); NS, not significant.
