Extended Data Fig. 10: TH1-like Treg cells do not impact tissue resident memory CD8+ T during IAV infection rechallenge.
From: IFNγ-induction of TH1-like regulatory T cells controls antiviral responses
a-g, Foxp3Cre-YFP or Ifngr1L/LFoxp3Cre-YFP mice were infected with PR8 only (n = 9, control; n = 8, Ifngr1-deficient) or X31 and challenged with PR8 (n = 10, control; n = 9, Ifngr1-deficient). a, Experimental schema. b, Expression of LAP/TGFβ1 by lung Treg cells by percent (upper) and level of expression within LAP/TGFβ1+ Treg cells (lower) were determined by flow cytometry. c, Percentage (upper), and number (lower), of lung IAV pooled Tetramer+ (PA224 and NP366) CD8+ T were determined by flow cytometry. d, Expression of IFNγ by lung CD8+ T cells was measured, after in vitro stimulation with pooled IAV peptides (PA224-233 and NP366-372), by flow cytometry. e, Expression of CXCR3 by lung pooled Tetramer+ CD8+ T cells was measured by flow cytometry. f, Percentage (left), and number (lower), of CD103+CD69+ lung pooled Tetramer+ CD8+ T cells were determined by flow cytometry. g, Blinded histology scores (areas indicated) of paraffin-embedded hematoxylin and eosin stained, lung tissue sections. b-g, Data are presented as mean values and represent biologically independent mice and 3 independent experiments. Statistical significance was determined by multiple unpaired Student’s t-tests relative to Foxp3Cre-YFP mice (P values indicated when significant); NS, not significant.
