Extended Data Fig. 2: TH1 signatures in Treg cells and CD4+ Tconv cells during acute and chronic infection.
From: IFNγ-induction of TH1-like regulatory T cells controls antiviral responses
a, Diagram of Il12rb2.Thy1.1 L/LhNGR targeted mouse. b, c, Il12rb2.Thy1.1 L/LhNGR mice (n = 11) were infected with Arm. and splenic Thy1.1– and Thy1.1+ CD4+ Tconv. cells purified and expression of Il12rb2 by Thy1.1– and Thy1.1+ CD4+ Tconv. cells was determined by qPCR (b). Thy1.1– and Thy1.1+ CD4+ Tconv. cells were serum starved and treated with PBS or IL-12, and induction of pSTAT4 was measured by flow cytometry (c). d, e, Foxp3Cre-YFP mice (n = 16, 5 independent experiments, Uninfected; n = 12, Arm.; n = 14, Cl. 13) remained uninfected or were infected with Arm. or Cl. 13, expression of Il12rb2 mRNA (d) and Ifngr1 mRNA (e) by sorted splenic CD4+ Tconv cells and Treg cells, without stimulation, were measured by qPCR. f-i, Expression of IFNGR1 (f) (n = 7, 2 independent experiments, Uninfected; n = 9, 2 independent experiments, Arm.; n = 15, Cl. 13), T-bet (g) (n = 7, Uninfected; n = 11, Arm.; n = 8, Cl. 13), CXCR3 (h) (n = 11, Uninfected; n = 9, 2 independent experiments, Arm.; n = 15, Cl. 13), T-bet (n = 7, 2 independent experiments, Uninfected; n = 11, 2 independent experiments, Arm.; n = 16, Cl. 13), GATA-3 (n = 6, 2 independent experiments, Uninfected; n = 9, 2 independent experiments, Arm.; n = 17, Cl. 13), and RORγt (n = 3, 2 independent experiments, Uninfected; n = 11, 2 independent experiments, Arm.; n = 8, 2 independent experiments, Cl. 13), (i) by splenic CD4+ Tconv cells and Treg cells were determined flow cytometry. b-i, Data are presented as mean values and represent biologically independent mice and 2 (g, and as indicated) or 3 (b-f, h, i) or 5 (as indicated) independent experiments. Statistical significance was determined by an unpaired two-tailed Student’s t-test relative to Thy1.1– (b) or CD4+ Tconv cells (f-h), or by a two-way AVOVA with multiple comparisons (c), or by a two-way ANOVA with multiple comparisons (d-e) and multiple unpaired t-tests relative to CD4+ Tconv cells (d, e, i) (P values indicated when significant); NS, not significant.
