Fig. 7: Early during MCMV infection pDCs are recruited at the MZ where they contact infected cells.
a–f, Spleen cryosections, 20 μm, from pDC-Tom mice stained with anti-tdT (magenta), anti-CD169 (white) and anti-CD3 (green) antibodies (a–c) or with anti-tdT (magenta), anti-CD169 (white) and anti-IE1 (cyan) antibodies (d–f). a, Representative images for NI or 12-h, 18-h and 24-h MCMV infection conditions. b, Microanatomical distribution of pDCs across the different areas of the spleen, during the course of MCMV infection. The data shown are from six animals for uninfected mice, six animals for 12 h, nine for 18 h and 11 for 24 h, with one whole spleen section analyzed per mouse. c, Quantification of the proportion of pDCs in the MZ. The data are shown as mean ± s.e.m. One-way ANOVA was used for the statistical analysis: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. d, Representative images for 12-h, 36-h and 48-h MCMV infection conditions. e, Microanatomical distribution of IE1+ cells across the different areas of the spleen, during the course of MCMV infection. The data shown are from two animals for each time point analyzed, with one whole spleen section analyzed per mouse. f, Number of IE1+ cells mm−2 quantified in the whole spleen section. The data are shown as mean ± s.e.m.
