Extended Data Fig. 4: TFAM regulates B cell clonality.

(a) Quantification of somatic hypermutation by Igh mutation count for indicated immunoglobulin isotype across all sequenced B cells in which isotype call could be made. Data are as described in Fig. 4a (IgG1 = 53 cells, IgG2b = 116 cells, IgG3 = 50 cells, IgM = 1038 cells, pooled from n = 3 Aicda-WT and n = 3 Aicda-Tfam mice). (b) Quantification of overall mutation rate for Ighv1-72 gene segment (n = 76 cells in Aicda-WT, n = 89 in Aicda-Tfam, pooled from n = 3 Aicda-WT and n = 3 Aicda-Tfam mice). (c) Amino acid substitution rate across Ighv1-72 in GC B cell cluster for Aicda-WT and Aicda-Tfam mice (n = 76 cells in Aicda-WT, n = 89 in Aicda-Tfam, pooled from n = 3 Aicda-WT and n = 3 Aicda-Tfam mice). (d) Amino acid substitution rate across Ighv1-72 in AP B cell cluster for Aicda-WT and Aicda-Tfam mice (n = 24 cells in Aicda-WT, n = 154 in Aicda-Tfam, pooled from n = 3 Aicda-WT and n = 3 Aicda-Tfam mice). Statistical significance was calculated by two-tailed t-test with correction for multiple comparison by the Benjamini-Hochberg method(a), or two-tailed unpaired t-test (b). In a,b the box and whisker plots depicts the minimum and maximum values no greater than ±1.5 × the IQR, the lower and upper quartiles, and the median.