Extended Data Fig. 6: ScRNA-seq analysis of tonsillar TRCs and immune cells during homeostasis and inflammation.

a, Gene sets enriched in TRCs according to GO enrichment analysis based on differentially expressed genes in adult TRCs compared to pediatric TRCs. b, Gene sets enriched in TRCs upon inflammation according to GO enrichment analysis based on differentially expressed genes in TRCs between adult patients with tonsillitis and OSA. Genes with an average log2 fold change (Avg. log2 FC) > 1 assigned to enriched terms are highlighted. c-g, ScRNA-seq analysis of the human palatine tonsil T and B cell landscape. c, Representative flow cytometric plots depicting the sorting strategy of CD3⁺ T cells and CD19⁺ B cells from human palatine tonsils according to the indicated markers. UMAP visualizations show T and B cells from adult patients with OSA and tonsillitis colored according to unbiased clustering (d), condition (e), and assigned B and T cell subsets (f). g, Dot plot depicts marker genes used for characterization and assignment of T and B cell subsets. ScRNA-seq data represents a total of 81,997 T and B cells from n = 2 adult patients with OSA and n = 3 adult patients with tonsillitis. Tcm, central memory T cells; Treg, regulatory T cells; Tfh, T follicular helper cells; CTL, cytotoxic T lymphocytes; GC, germinal center; MBC, memory B cells; PC, plasma cells.