Extended Data Fig. 9: Co-culture of PI16⁺ RCs with T cells.

a–e, Carboxyfluorescein succinimidyl ester (CFSE)-labeled tonsillar T cells from adult patients with OSA or tonsillitis were cultured for 3 days with PI16⁺ RCs and indicated recombinant proteins and were stimulated with phytohemagglutinin (PHA). a, Representative flow cytometric plots depicting CD25 expression on CFSE^low CD8⁺ T cells. b, Quantification of CFSE^low cells as a percentage of CD8⁺ T cells. c, Quantification of CD25⁺ cells as a percentage of CFSE^low CD8⁺ T cells. d, CD25 mean fluorescence intensity (MFI) fold change of CFSE^low CD25⁺ CD4⁺ T cells. e, IL-6 concentration in culture supernatant. b–e, Mean and s.d. are indicated, n = 4 patients. Q values were calculated with the repeated measure (RM) one-way ANOVA (b, c, e) or Ordinary one-way ANOVA (d) test and Benjamini, Krieger and Yekutieli correction for multiple comparisons.