Extended Data Fig. 3: Flow cytometric and scRNA-seq analysis of stromal cells in human palatine tonsils. | Nature Immunology

Extended Data Fig. 3: Flow cytometric and scRNA-seq analysis of stromal cells in human palatine tonsils.

From: PI16+ reticular cells in human palatine tonsils govern T cell activity in distinct subepithelial niches

Extended Data Fig. 3

a,b, Representative flow cytometric plots depicting the gating strategy for each stromal cell type according to the indicated markers. c, Quantification of epithelial cells (EpC) as a percentage of total CD45– CD235a– live cells in pediatric (n = 5) and adult (n = 6) patients with OSA. Data show average value of left and right tonsils for each patient. Mean and s.d. are indicated. P value was calculated with the two-sided Mann-Whitney test. d, Representative flow cytometric plots depicting the sorting strategy for scRNA-seq of CD45– CD235a– stromal cells from human palatine tonsils according to the indicated markers. e–g, Expression patterns of indicated BEC, LEC and EpC gene signatures projected onto UMAPs. ScRNA-seq data represents a total of 86,966 CD45– CD235a– stromal cells from n = 12 patients.

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