Extended Data Fig. 1: CD40 synergizes with varying inflammatory stimuli BMDC1.
From: CD4+ T cell calibration of antigen-presenting cells optimizes antiviral CD8+ T cell immunity
a, ‘BMDC1-IFN-αA+CD40’ increase secretion of CCL4, TNF-α and CCL5 (from left to right) over time compared to ‘BMDC1-IFN-αA’, ‘BMDC1-CD40’ and ‘BMDC1-unstimulated’. Data are presented as mean ± s.e.m pooled from 3 independent experiments. Adjusted p-value of statistically significant differences between conditions as assessed by one-way ANOVA indicated. b, Changes in Il15 and Cxcl16 expression in ‘BMDC1-IFN-αA+CD40’ and ‘BMDC1-IFN-β+CD40’ compared to ‘BMDC1-IFN-αA’ or ‘BMDC1-IFN-β’ respectively and to ‘BMDC1-CD40’ and ‘BMDC1-unstimulated’. c. Tnf and Ccl4 in expression in BMDC1s stimulated with LPS, CpG or poly(I:C) for 6 h with or without CD40 Ab for the last 30 min. b-c, Data are presented as mean ± s.e.m pooled from 3 independent experiments. Adjusted p-value of statistically significant differences between conditions as assessed by one-way ANOVA indicated; ns = non-significant. d, Percent of MHC-IIhi CD8+ DCs from IFNαR-deficient (Ifnar2−/−) and WT mice naïve or 2 days after epicutaneous HSV-1 infection. Data are presented as mean ± s.e.m pooled from 7 independent experiments (n≥5 per experiment). Statistically significant differences between conditions as assessed by Mann-Whitney test; two-tailed p-value indicated; ns = non-significant. e. ‘BMDC1-IFN-αA’ and ‘BMDC1-unstimulated’ increase CD40 expression to comparable levels over time. Data are presented as mean ± s.e.m pooled from 3 independent experiments. Two-way ANOVA performed between the corresponding conditions ns = non-significant.
