Extended Data Fig. 1: scRNA-seq analysis of FLS isolated from RA synovium.
From: Drivers of heterogeneity in synovial fibroblasts in rheumatoid arthritis
a, Gating strategy for FACS sorting of FLS to exclude PDPN- mural cells. Numbers below population are percentage of parent population. b, Numbers of UMI counts per cluster. c, Cell cycle G2M score per cluster. d, Force directed layout without batch correction colored by cluster (left) and patient (right). e, Cluster composition by patient after Harmony batch correction. f, Dotplot showing the relative per cluster expression of previously published cluster-derived gene signatures: Zhang et al2 above and Alivernini et al4 below horizontal line. g, Representative confocal image of PDPN (red), THY1 (orange), CD34 (white), CD31 (green), CD3 (magenta) and nuclear marker (blue) from RA synovial tissue (n = 4 tissues). White arrow indicates individual CD34+ THY1+PDPNlowCD31− cell. h, Trajectory analysis using Palantir starting from cells in either the activated (left) or resting (right) lining states (starting point marked with a star).
