Extended Data Fig. 7: Effect of Gnas deletion CD8 T cells in mice bearing 4MOSC1 tumors.
From: The GPCR–Gαs–PKA signaling axis promotes T cell dysfunction and cancer immunotherapy failure
a, Tumor growth curve (left panel) and quantification of endpoint tumor volume (right panel) of CD8-Gnas+/+ (n = 14 mice) and CD8-Gnas−/− mice ( = 12 mice) implanted with 4MOSC1 tumors. Mice were given 3 doses of tamoxifen prior to orthotopic tumor implantation. The average tumor volume and s.e.m. are shown. Statistical significance was determined by two-way ANOVA. b, Quantification of PD-1+TIGIT+ CD8 T cells in 4MOSC1 tumors and draining lymph nodes at endpoint. The average frequency and s.e.m. are shown (n = 5 mice per group). Statistical significance was determined by two-tailed unpaired Student’s t-test. c, Frequency of CD8+ T cells in 4MOSC1 tumors in CD8-Gnas KO mice versus littermate controls. The average frequency and s.e.m. are shown (n = 5 mice per group). Statistical significance was determined by two-tailed unpaired Student’s t-test.
