Extended Data Fig. 8: Gating strategy for chronic stimulation and in vivo experiments.
From: The GPCR–Gαs–PKA signaling axis promotes T cell dysfunction and cancer immunotherapy failure
a, For chronic stimulation experiments, lymphocytes were gated from forward scatter area (FSC-A) and side scatter area (SSC-A). Single cells were distinguished from doublet cells in forward scatter height (FSC-H) and forward scatter width (FSC-W), and then side scatter height (SSC-H) and side scatter width (SSC-W). Live CD8 cells were then gated. b, For in vivo experiments, lymphocytes were gated from forward scatter area (FSC-A) and side scatter area (SSC-A). Single cells were distinguished from doublet cells in forward scatter height (FSC-H) and forward scatter width (FSC-W), and then side scatter height (SSC-H) and side scatter width (SSC-W). Live CD45 cells were then gated. T cells were distinguished by NK1.1 negative, CD19 negative, and CD3 positive. CD8 T cells were then gated as CD4 negative.
