Fig. 5: TCR- and cytokine-activated MAIT cells follow distinct linear trajectories.
From: Single-cell analysis of human MAIT cell transcriptional, functional and clonal diversity
a,b, UMAPs of MAIT cells from all donors colored by stimulation condition (a) or the nine identified clusters (b). n = 27,305 cells from three donors. c, Proportion of cells in each cluster from the three stimulation conditions. d, Heatmap showing row-scaled log-transformed normalized expression of the top five marker genes for each cluster. e, UMAPs colored by expression of IFNG, IL26, CCL4 and GZMB. f, UMAP of MAIT cells from all donors with the branching pseudotime trajectory identified using Slingshot shown in black. g, Spearman’s rank correlation between gene importance (log2 1/gene importance rank) on SCORPIUS TCR and cytokine trajectories. Labels indicate the most differentially important genes, ten with higher importance on the TCR trajectory and ten with higher importance on the cytokine trajectory. Colors indicate whether gene expression was upregulated (red), downregulated (blue) or mixed (purple; upregulated in TCR and downregulated in cytokine or vice versa) relative to unstimulated cells. h,i, Expression of IL26, CCL3 and TNFRSF9 along SCORPIUS TCR (h) and cytokine (i) trajectories.
