Extended Data Fig. 1: Sorting and characterization of positively selecting thymocytes. | Nature Immunology

Extended Data Fig. 1: Sorting and characterization of positively selecting thymocytes.

From: Single-cell multiomic analysis of thymocyte development reveals drivers of CD4+ T cell and CD8+ T cell lineage commitment

Extended Data Fig. 1: Sorting and characterization of positively selecting thymocytes.

a, Representative FACS plots displaying gating strategy to sort thymocytes for CITE-seq. Cell populations were gated and sorted to include lymphocytes, exclude forward scatter doublets, include Ghost Dye Violet 510 Live/Dead stain negative (live cells), then on TCRβ+CD5+ to enrich for cells that were positively selecting. b-c, Heatmaps of manually selected cell-type markers for RNA (b) and proteins (c). Values are totalVI denoised expression. d, UMAP plot of totalVI latent space from positively selected thymocytes before filtering indicating annotated populations that were retained (positively selecting thymocytes) or removed (all other populations) from downstream analysis.

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