Extended Data Fig. 3: Systemic effects of FD consumption are not mediated by APCs.
From: Short-term dietary changes can result in mucosal and systemic immune depression
(a) Dietary intervention and immunization strategy. (b) Representative histograms (left) and expression level (right) of MHC class II gated on CD19+ B, CD11c+ pDCs, CD11c+CD11b− cDC1 and CD11c+CD11b+ cDC2 cells isolated from draining LNs of RD- and FD-fed mice. (c) Representative histograms (left) and expression level (right) of CD80, CD86 and CD40 gated on CD11c+CD11b− cDC1 cells isolated from draining LNs of RD- and FD-fed mice. (d) Representative histograms (left) and expression level (right) of CD80, CD86 and CD40 gated on CD11c+CD11b+ cDC2 cells isolated from draining LNs RD- and FD-fed mice. (e) Dietary intervention and immunization strategy. (f) (Left) Quantification of footpad swelling of recipient mice that had received 1 × 106 OT-II cells and had been fed RD or FD starting from the day of the adoptive transfer. A group of recipient mice received PBS alone as control. (Right) Representative pictures of swollen footpads of recipient mice. Data in (B-D) are a pool of 2 experiments (n = 7 each). Data in (F) are a pool of 2 experiments (n = 10, 9 or 8). Data are shown as mean ± SEM. P-values were determined using a Brown-Forsythe and Welch ANOVA test with Holm-Sidak’s multiple comparisons test.
