Extended Data Fig. 4: Effects of FD are not dependent on fat and calorie-intake.
From: Short-term dietary changes can result in mucosal and systemic immune depression
(a) Body weight normalized on day 0 of mice fed RD and FD ad libitum or pair-fed. (b) Serum levels of cholesterol (mg/dll) of mice kept on RD or switched to FD ad libitum or pair-fed. (c) Expression levels of Il17a and Il17f normalized to Tbp and shown as relative to RD, as measured by RT-PCR on total PP (left) and ileum (right) cells isolated from RD-, FD- and FD2-fed mice. (d) Representative dot plots (left) and frequencies (right) of PP TH17 cells among Tconv cells isolated from mice fed a low-fat, fiber-deprived diet (FPD) or low-fat, 30% inulin-added high-fiber diet (FRD). Data in (A-B) are from one experiment (n = 5 each).Data in (C) are a pool of 2 experiments (PPs, n = 8 or 9) and from one experiment, representative of two (ileum, n = 5 each). Data in (D) are a pool of 2 experiments (n = 8 each). Data are shown as mean ± SEM. P.values have been determined by Kruskal-Wallis test with Dunn’s multiple comparisons test (B-C) or two-tailed non-parametric Mann-Whitney test (D).
