Extended Data Fig. 5: CD71 ablation in ILC3s impairs gut immunity to C. rodentium infection (Related to Fig. 5).
From: Nutrition impact on ILC3 maintenance and function centers on a cell-intrinsic CD71–iron axis
Body weight (a) and survival (b) of C. rodentium-infected mice (Tfrcf/f: n = 8; Tfrcf/f Rorc-cre: n = 5). Statistical analysis by the log-rank test. Data are compiled from three independent experiments. c, TH17 percentages in CD4+ T cells in C. rodentium-infected mice from two independent experiments (for each time point: Tfrcf/f: n = 8 and 6; Tfrcf/f Rorc-cre: n = 4). d and e, FACS analysis of CD71 in ILC3s of WT (Tfrcf/f) mice treated with or without iron dextran. Representative FACS plot (d) and percentages of CD71+ cells in ILC3s from three independent experiments (n = 5 for untreated; n = 7 for treated) (e). f, Heat map of OXPHOS pathway and ILC3 signature genes by RNA-seq analysis of SI ILC3s (n = 3 mice per group). g, GSEA showing glycolysis gene pathway enrichment in the indicated ILC3s. h, Representative FACS plot of pS6 in ILC3s of three independent experiments. i–k, FACS analysis of mitochondrial mass and activity. gMFI of MitoTracker Green FM (i) (n = 3 mice per group), TMRE (j) (Tfrcf/f: n = 6; Tfrcf/f Rorc-cre: n = 4), MitoSox (k) (Tfrcf/f: n = 6; Tfrcf/f Rorc-cre: n = 5) in ILC3s from three independent experiments. l–q, RNA-seq analysis of SI ILC3s from mice with or without iron dextran treatment (n = 3 mice per group). Heat map of the DEGs by RNA-seq analysis in the indicated ILC3s shown in Fig. 5h (l). GSEA showing ILC3 signature gene enrichment (m). FPKM of Ahrr, Gem and Osgin1 in the indicated ILC3s (n–p) (n = 3 mice per group). GO analysis showing enriched top 10 pathways of the shared 235 DEGs (cKO vs WT, with or without iron dextran) shown in Fig. 5h (q).
