Extended Data Fig. 9: Iron treatment alleviates ILC3 defects in Ahr-deficient mice (Related to Fig. 6).
From: Nutrition impact on ILC3 maintenance and function centers on a cell-intrinsic CD71–iron axis
a and b, LI-ILC3 percentages in Lin− LPLs (a) and absolute numbers (b) in the indicated mice with or without iron dextran treatment. Data are compiled from three independent experiments (Ahr+/+: n = 7 and 8 for untreated and treated; Ahr−/−: n = 5 and 6 for untreated and treated). c–e, FACS analysis of IL-22 and IL-17A expression in SI ILC3s of the indicated mice. Representative FACS plot (c), IL-22+ (d) and IL-17A+ (e) percentages in the indicated ILC3s from three independent experiments (Ahr+/+: n = 7 and 8 for untreated and treated; Ahr−/−: n = 5 and 6 for untreated and treated). f and g, FACS analysis of RORγt in Lin− LPLs of the mice with indicated treatment. Representative FACS plot (f) and LI-ILC3 percentages in Lin− LPLs from three independent experiments (g) (Ahrf/f: n = 6 and 5 for untreated and treated; Ahrf/f Rorc-cre: n = 6 mice per group). h and i, Numbers of CCR6+ ILC3s (h) and NKp46+ ILC3s (i) in SI LPLs of the indicated mice from three independent experiments (Ahrf/f: n = 6 and 5 for untreated and treated; Ahrf/f Rorc-cre: n = 6 mice per group). j, Cryptopatches (CPs)/intestinal lymphoid follicles (ILFs) in representative SI sections of the indicated mice were stained with RORγt (red), CD3 (green), and DAPI (blue) and analyzed by fluorescence microscopy. Scale bar, 100 µm. Data are representative of two independent experiments. k, Numbers of CPs/ILFs in the SI of the indicated mice (n = 3 mice per group compiled from two independent experiments). l, Percentages of Aqua+ in SI ILC3s from three independent experiments (Ahrf/f: n = 6 and 5 for untreated and treated; Ahrf/f Rorc-cre: n = 6 mice per group).
