Extended Data Fig. 2: Iron chelation represses ILC3 functions ex vivo and in vitro (Related to Fig. 2).
From: Nutrition impact on ILC3 maintenance and function centers on a cell-intrinsic CD71–iron axis
a–l, SI LPLs from WT mice were treated ex vivo with or without DFO for 16 h. Percentages of IFN-γ+ in ILC3s compiled from two independent experiments (n = 6 mice per group) (a), gMFI of MitoTracker Green FM (b), TMRE (c) and MitoSox (d) in ILC3s (n = 6 mice per group from two independent experiments). FACS analysis of CD71 expression in ILC2s and representative FACS plot of three independent experiments (e). Representative FACS plots (f), percentages of IL-5+ (g) and IL-13+ (h) cells in ILC2s compiled from two independent experiments (n = 6 mice per group) are shown. FACS analysis of CD71 expression in ILC1s and representative FACS plot of three independent experiments (i). Percentages of IFN-γ+ cells in ILC1s (j), IL-22+ (k) and IL-17A+ (l) cells in CD4+ T cells compiled from three independent experiments (n = 6 mice per group). m–s, LI LPLs from WT mice were treated ex vivo with or without DFO for 16 h. FACS analysis of CD71 (m) and RORγt (o) expression in ILC3s and representative FACS plots of three independent experiments are shown. gMFI of CD71 (n) and RORγt (p) expression in ILC3s from three independent experiments (n = 3 mice per group) are shown. FACS analysis of IL-22 and IL-17A expression in ILC3s and representative FACS plot of three independent experiments (q). Percentages of IL-22+ (r) and IL-17A+ (s) in ILC3s from three independent experiments (n = 3 mice per group). t and u, MNK-3 cells were treated with or without DFO for 16 h in vitro. gMFI of Ki-67 expression in MNK-3 cells (t) and percentages of MNK-3 cell viability (u) (n = 8 biologically independent replicates per group from three independent experiments).
