Extended Data Fig. 5: CD8+ T cell responses require GOT1 during acute infection.
From: The malate shuttle detoxifies ammonia in exhausted T cells by producing 2-ketoglutarate
a, Experimental design illustration. b, FACS gating strategies used in c–l. c–l, Contour plots, histograms, and bar graphs show the flow cytometry staining results of Ly5.1+ donor P14 CD8+ T cells (c,d), KLRG1 and IL-7Rα (e,f), cytokines (g,h), Ki-67 (i,j), and cleaved caspase-3 and Bim (k–l) in Got1-deficient and sufficient P14 CD8+ T cells. Cells were stimulated with GP33–41 peptide before the flow cytometry staining (g,h). Data were pooled from two independent experiments (c–l) with ten C57BL/6 mice in each group receiving Got1-deficient and sufficient donor P14 CD8+ T cells. The results are presented as mean ± s.d. **P<0.01; ***P<0.001; ****P<0.0001; n.s., not significant. Comparisons were performed using a two-tailed Student’s t-test (c, d, %KLRG1−IL-7Ra+ T cells among donor T cells in e, f, g, h, i, j, k, l; data points were normally distributed) or the two-tailed Mann-Whitney test (%KLRG1+IL-7Ra− T cells among donor T cells in e; data points were not normally distributed). P values in c: 1.6 × 10−5, 0.006; in d: 0.675, 0.745; in e: 0.752, 0.397; in f: 0.127, 0.738; in g: 6.4 × 10−6; in h: 0.069; in i: 2.4 × 10−4; in j: 0.3959; in k: 1.1 × 10−5, <1.0 × 10−15; in l: 0.194, 0.088. Six-week-old female mice were used (b–l).
