Fig. 2: Antiviral CD8+ T cell responses require GOT1 during chronic infection.
From: The malate shuttle detoxifies ammonia in exhausted T cells by producing 2-ketoglutarate
a, Illustration of the experimental design. b, Fluorescence-activated cell sorting (FACS) gating strategies used in c–l. c–l, Contour plots, histograms and bar graphs show the flow cytometry staining results of Ly5.1+ donor P14 CD8+ T cells (c,d), PD-1 and TIGIT (e,f), cytokines (g,h), Ki-67 (i,j) and cleaved caspase-3 and Bim (k,l) in Got1-deficient and sufficient P14 CD8+ T cells. Cells were stimulated with GP33–41 peptides before the flow cytometry staining (g–h). Data were pooled from two independent experiments (c–l) with ten C57BL/6 mice in each group receiving Got1-deficient and sufficient donor P14 CD8+ T cells. The results are presented as mean ± s.d. **P < 0.01; ***P < 0.001; ****P < 0.0001. Comparisons were performed with the two-tailed Mann–Whitney test (percentage of Ly5.1+ donor cells in d and i, cleaved caspase-3 MFI in k and Bim MFI in l; data points were not normally distributed) or a two-tailed Student’s t-test (other comparisons). In c, P = 1.14 × 10−10 (left) and P = 9.48 × 10−12 (right); in d, P = 1.08 × 10−5 (left) and P = 9.08 × 10−7 (right); in e, P = 0.0048; in f, P = 1.0 × 10−15; in g, P = 2.47 × 10−5; in h, P = 6.99 × 10−6; in i, P = 2.17 × 10−5; in j, P = 0.0003; in k, P = 1.08 × 10−5 (left) and P = 8.84 × 10−11 (right); in l, P = 8.70 × 10−9 (left) and P = 1.08 × 10−5 (right). Six-week-old female mice were used (b–l).
