Fig. 6: 2-KG restores Got1-deficient CD8+ T cell antiviral responses in vivo.
From: The malate shuttle detoxifies ammonia in exhausted T cells by producing 2-ketoglutarate
a, Experimental design. b–f, Contour plots, histograms and bar graphs show the flow cytometry staining results of Ly5.1+ donor P14 CD8+ T cells (b), PD-1 and TIGIT (c), cytokines (d), Ki-67 (e), and cleaved caspase-3 and Bim (f) in Got1-deficient and sufficient P14 CD8+ T cells. Cells were stimulated with GP33–41 peptides before flow cytometry staining (d). g,h, Bar graphs show the viral titers at day 8 (g) and day 30 (h) after infections. Data were pooled from two independent experiments (b–h). n = 10 C57BL/6 mice per group. The results are presented as mean ± s.d. **P < 0.01; ***P < 0.001; ****P < 0.0001. Comparisons were performed with one-way ANOVA. In b, P = 1.6 × 10−9, P = 3.2 × 10−11, P = 1.3 × 10−7 and P = 5.8 × 10−7; in c, both P = 1.1 × 10−14; in d, P = 4.5 × 10−14 and P = 1.1 × 10−11; in e, P = 2.5 × 10−6 and P = 0.00015972; in f, P = 1.7 × 10−14, P = 1.9 × 10−14, P = 3.7 × 10−9 and P = 4.0 × 10−9; in g, P = 0.937 and P = 0.477; in h, P = 0.002629596 and P = 0.000658958. Six-week-old female mice were used (b–h).
