Extended Data Fig. 2: NFAT1 binds to Got1 locus.
From: The malate shuttle detoxifies ammonia in exhausted T cells by producing 2-ketoglutarate
a–d, P14 splenocytes were cultured with GP33–41 peptide for 3 days before ChIP-PCR analysis. PCR was performed using primers evenly spaced across a 10kb region of Got1 locus. Line curves show the relative quantification results of DNA fragments pulled down using indicated antibodies or IgG isotype control. Bar graphs show the binding of NFAT1 to Il2 (a known target gene of NFAT1) locus, the binding of TOX to Pdcd1 (a known target gene of TOX) locus, the binding of Eomes to Il2rb (a known target gene of Eomes) locus, and the binding of Blimp1 to Id3 (a known target gene of blimp1) locus. The data are presented as mean ± s.d. **P<0.01; ***P<0.001; ****P<0.0001. Comparisons were performed with two-way ANOVA (line graphs, a–d), a two-tailed Student’s t-test (bar graphs, a–d, data points were normally distributed). P values in a (from left to right): 9.1 × 10−13, <1.0 × 10−15, <1.0 × 10−15, 0.002; b: 0.0003; c: 0.0003; in d: 0.0002. Seven-week-old male mice were used (a–d).
