Extended Data Fig. 6: ADT expression profiles of MNP-2 CD8 T cells.

(a) Expression heatmap of 36 ADTs across all CD8 T cell subsets in our TEA-seq dataset. Lineage markers CD3, CD4, CD8a, and CD16 were excluded from analysis. For visualization, values have been scaled (z-score) for each marker. (b) Single cell expression of surface protein ADTs CD27, CD244, and CD11b on MNP-2, true naive and TEMRA CD8 T cell subsets pooled from all donors (n = 16) in our TEA-seq dataset. Box plots are 25% and 75% quartiles with median shown. c) Protein expression of CD244 and CD11b on TCRab⁺ CD8⁺CD4^neg T cells in cord blood (CB) and older adult (OA) PBMCs determined by flow cytometry. (d) Average RNA expression of CD8aa-specific genes in CD244⁺CD11b⁺ and CD244^negCD11b^neg populations of TCRab⁺CD8⁺CD4^neg T cells sorted from cord blood (n = 4 donors) determined by qRT-PCR. (e) Surface protein expression of CD8A, CD8B, CD27, and IL-21R on CD244+CD11b+ and CD244^negCD11b^neg populations of TCRab⁺ CD8⁺CD4^neg T cells from cord blood (n = 3 donors). P-values were determined by two-tailed paired t-test. *P < 0.05 (p = 0.028), **P < 0.01 (P = 0.0016), ns = not significant.

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