Extended Data Fig. 3: Stromal remodeling of the TME.

(a) FACS gating strategy for S1, S2 and S3 stromal populations from MO5 tumors. (b) Normalized expression of the indicated genes in stromal populations from melanomas single cells RNAseq⁹. (c) Expression of the indicated genes, measured by qRT-PCR, in S1, S2 and S3 isolated from MO5 tumors as shown in a (n = 3). (d) Percentage of the indicated stromal populations, measured by FACS, in MO5 tumors from ADAM12-DTR⁺ (DTR) and DTR^– littermates (Ctrl) treated with DT as in Fig. 2a (n = 4). (e) Normalized expression of Adam12 from single cells RNAseq of melanomas and normal skin⁹. (f) FACS plot and percentages of GFP⁺ cells in the total stromal fraction from MO5 tumors. One representative experiment from independent experiments is shown (n = 7). (g) Expression of the indicated genes, measured by qRT-PCR, in stromal cells treated as indicated; n = 3. Gating strategy for stromal populations S1: PDGFRα^High PDPN^High CD34^High DPP4^High Ly6c^High; S2: PDGFRα⁺ PDPN⁺ CD34^Mid/Low DPP4^Low/–; S3: PDGFRα^Low PDPN^Low CD34^Low DPP4^–. Statistics were calculated using two-tailed, unpaired Student’s t-test (d,g). Data are presented as mean values +/– SD.

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