Extended Data Fig. 6: B cells from DENV-infected patients harbor replicating virus.

a, Stack bar plots showing the absolute numbers and distribution of VHCs across cell types in each of the 10 patients with detectable vRNA. b, Cumulative distributions of DENV reads/million reads per cell in B cell subtypes. p values by KS test followed by Bonferroni correction between distinct B cell subtypes are indicated. c, Flow cytometry gating strategy used to define B cells (CD19⁺), T cells (CD3⁺), classical monocytes (CD14⁺CD16⁻), nonclassical monocytes (CD14⁻CD16⁺), intermediate monocytes (CD14⁺CD16⁺), CD56^bright NK cells, CD56^dimCD16⁺ NK cells, pDCs (CD123⁺CD303⁺), cDC1s (CD141⁺) and cDC2s (CD1c⁺). d, Distributions of intracellular expression of DENV envelope (E) protein measured via spectral flow cytometry in B cells (CD19⁺), classical monocytes (CD14⁺CD16⁻), nonclassical monocytes (CD14⁻CD16⁺), intermediate monocytes (CD14⁺CD16⁺), cDC1s (CD141⁺), cDC2s (CD1c⁺), pDCs (CD123⁺CD303⁺), CD56^bright NK cells, CD56^dimCD16⁺ NK cells, and T cells from the indicated DENV-infected patients (n = 6 participants from two independent experiments) with viremia ranging from 10⁵ to 10⁹ viral copies/mL. e, Number of negative strand RNA-harboring cells (VHCs) over total number of VHCs across immune cell types in three DWS patients with highest vRNA reads shown in Fig. 4a. f, Dot plot showing the average ratio of negative strand over total strand of housekeeping genes (gray) and DENV RNA (orange) reads within the 20 single cells with the largest DENV read counts in three DWS patients with highest vRNA reads shown in Fig. 4a.