Extended Data Fig. 2: All CARs containing the TCR-CD3 ICD deliver tonic signals.
a, Activation markers (n = 4 for CD69, n = 5 for CD25, n = 6 for 4-1BB), b, endogenous TCR-CD3 expression (n = 4), c, differentiation (n = 4) and d, CD4/CD8 ratios (n = 3) of CAR T cells 6 days after transduction measured by flow cytometry. Each dot represents an independent donor. TCM, central memory; TEM, effector memory; and TEMRA, effector memory RA. Data are represented as mean ± s.d. One-way ANOVA (a, b) or Two-way ANOVA (c, d), followed by Dunnett’s multiple comparisons test. All comparisons to BBζ were non-significant.
